Biology Project Abstract
THE KIRBY-BAUER ASSAY IN RELATIONSHIP TO STUDENT INQUIRY
Presenter:
Nan Sethakorn, Illinois Mathematics and Science Academy, 1500 W. Sullivan Road, Aurora, IL 60506; pbear@imsa.edu
Advisor:
Judith Scheppler, Ph.D., Coordinator of Student Inquiry, Illinois Mathematics and Science Academy, 1500 W. Sullivan Road, Aurora, IL 60506; 630-907-5899; quella@imsa.edu
Abstract:
Various natural substances were tested using a modification of the Kirby Bauer assay to determine whether they inhibit bacterial growth. The procedure involves plating an overnight culture of E. coli HB101, or other bacteria, on Luria-Bertani agar and then placing filter disks soaked in a solution of the test product onto the agar surface. After incubating the plates overnight, they are observed for zones of growth inhibition around the disks. From this project, garlic, cloves, madras curry powder, tea tree oil, and Sunbreeze oil (a manufactured oil containing cassia, eucalyptus, and peppermint oils) were found to inhibit bacterial growth. This inquiry also involves writing an article on how this modified Kirby Bauer assay can be used to promote student inquiry. For example, students can choose which bacteria and which substances to test, thereby designing their own experiments. Test substances can include but are not limited to spices, essential oils, citrus fruit extracts, other plant extracts, and cleaning products. The goal is to spark student interest in science, to promote scientific thinking, to increase comfort in experimentation, and to enhance laboratory skills. This project will culminate in an article that will be submitted to a science teaching journal for publication.